yloH [2019-07-05 12:42:30]

omega subunit of [SW|RNA polymerase]

locus: BSU15690

pI: 5.51

mw: 7.62 kDa

protein length: 67 aa

gene length: 204 bp

function: unknown

product: omega subunit of [SW|RNA polymerase]

essential: no

ec: 2.7.7.6

synonyms: rpoZ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]

Gene

Coordinates: 1,642,567 1,642,770

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)

Protein family

• [SW|RNA polymerase] subunit omega family (according to Swiss-Prot)

Structure

• [PDB|5UH5] (transcription initiation complex from Mycobacterium tuberculosis, 37% identity, aa 1- 52) [pubmed|28392175]

[SW|Localization]

• closely associated with [SW|RNA polymerase] involved in transcribing both mRNA and rRNA operons [Pubmed|20724389]

Expression and Regulation

Operons

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

  description

  • [pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|23396918], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed during vegetative growth and early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|23396918]

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|yloI]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description

  • [pubmed|22383849]

• genes: [gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]-[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

  description

  • [pubmed|22383849]

  regulation

  • expressed during sporulation in the mother cell [pubmed|12161109]

Biological materials

Mutant

• BKE15690 ([gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAATTGACGGATCTAACA, downstream forward: _UP4_TAGTAGCACAAGTAGCAACC
• BKK15690 ([gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAATTGACGGATCTAACA, downstream forward: _UP4_TAGTAGCACAAGTAGCAACC

References

Reviews

• 22210308,25878038

Original publications

• 11158566,20724389,26098117,28392175,27799328